- 询价
- BIO-RAD
- MCA1396D800GA
- 2025年10月17日
- WB
- Synthetic Peptide
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
PAX6 transcription factor linked to histidine tag.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to DyLight® 800 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
DyLight®800
- 适应物种：
Synthetic Peptide
- 保质期：
自发货之日起 12 个月
- 目录编号：
AD1.1.10
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
WB
- 浓度：
IgG concentration 1.0mg/ml
- 抗体名：
Histidine Tag antibody | AD1.1.10
- 规格：
0.1 mg
Mouse anti Histidine tag antibody, clone AD1.1.10 recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (
Hoffmann
et al. 2007) and
Hwang
et al. 2008) and non-mammalian (
Zheng
et al. 2007;
Gunnarsen
et al. 2010; and
de Vooght et al. 2012) cell lines.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | ✓ | 1/1000 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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文献和实验该产品被引用文献
References for Histidine Tag antibody
- Els Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs.
J Biol Chem. 276 (10): 7346-50. - Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice.
Immunol. 103: 301-309. - Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells.
J Biol Chem. 282 (31): 22370-5. - Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen.
BMC Immunol. 8: 4. - Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances *-, amphetamine- and *-induced reward and behavioral sensitization.
Genes Brain Behav. 7 (2): 244-56. - Wrighton, K.H. et al. (2009) Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors.
J Biol Chem. 284 (15): 9755-63. - Diefenbacher, M. et al. (2011) The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae.
J Biol Chem. 286: 25027-38. - Alvarez, M.M. et al. (2010) Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.
PLoS One. 5: e10176.
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文献支持
Mouse anti Histidine Tag:DyLight®800，clone AD1.1.10
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