- 询价
- BIO-RAD
- STAR117D550
- 2025年10月17日
- F, IF, WB
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Whole mouse IgG
- 亚型：
IgG
- 形态：
Purified IgG conjugated to DyLight 550 - liquid
- 克隆性：
Polyclonal Antibody
- 标记物：
DyLight®550
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
Polyclonal IgG
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F, IF, WB
- 浓度：
IgG concentration 1.0 mg/ml.
- 抗体名：
IgG antibody
- 规格：
0.1 mg
Goat anti Mouse IgG antibody recognizes mouse IgG and light chains common to other mouse immunoglobulin classes.
Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.
Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFα and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry (Redondo et al. 2013).
Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.
Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFα and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry (Redondo et al. 2013).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/50 | 1/400 |
| Immunofluorescence | ✓ | 1/50 | 1/400 |
| Western Blotting | ✓ | 1/500 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50μl of the suggested working dilution to label 1x106 cells in 100μl
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文献和实验该产品被引用文献
References for IgG antibody
- Banerjee, K. et al. (2012) Occluding the mannose moieties on * type 1 gp120 with griffithsin improves the antibody responses to both proteins in mice.
AIDS Res Hum Retroviruses. 28 (2): 206-14. - Abdala-Valencia, H. et al. (2012) Vitamin E isoforms differentially regulate intercellular adhesion molecule-1 activation of PKCα in human microvascular endothelial cells.
PLoS One. 7: e41054. - Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48. - Askari, N. et al. (2015) Tetracycline-regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse sciatic nerve regeneration upon transplantation.
Neuroscience. 305: 197-208. - Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
Vet Immunol Immunopathol. 164 (1-2): 87-92. - Singh, S.M. et al. (2016) Characterization of Immune Responses to an Inactivated Avian Influenza Virus Vaccine Adjuvanted with Nanoparticles Containing CpG ODN.
Viral Immunol. 29 (5): 269-75. - Alimolaei, M. et al. (2017) A Recombinant Probiotic, Lactobacillus casei, Expressing the Clostridium perfringens α-toxoid, as an Orally Vaccine Candidate Against Gas Gangrene and Necrotic Enteritis.
Probiotics Antimicrob Proteins. Apr 11 [Epub ahead of print]. - Topoluk, N. et al. (2017) Amniotic Mesenchymal Stromal Cells Exhibit Preferential Osteogenic and Chondrogenic Differentiation and Enhanced Matrix Production Compared With Adipose Mesenchymal Stromal Cells.
Am J Sports Med. 45 (11): 2637-46.
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Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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