Mouse anti Rat CD31:Biotin,clone TLD-3A12
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Mouse anti Rat CD31:Biotin,clo

ne TLD-3A12
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  • 询价
  • BIO-RAD已认证
  • MCA1334B
  • 2025年10月17日
  • F
  • Rat
  • 详细信息
  • 文献和实验
  • 技术资料
  • 免疫原

    Activated, Lewis rat derived microglial cells.

  • 亚型

    IgG1

  • 形态

    Purified IgG conjugated to biotin - liquid

  • 克隆性

    Monoclonal Antibody

  • 标记物

    Biotin

  • 适应物种

    Rat

  • 保质期

    自发货之日起 12 个月

  • 目录编号

    TLD-3A12

  • 供应商

    伯乐生命医学产品(上海)有限公司

  • 应用范围

    F

  • 浓度

    IgG concentration 0.1 mg/ml

  • 抗体名

    CD31 antibody | TLD-3A12

  • 规格

    0.1 mg

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.

Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009).

Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001)


储存信息

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.


应用

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl


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图标文献和实验
该产品被引用文献

Source Reference

  1. Flaris, N.A. et al. (1993) Characterization of microglia and macrophages in the central nervous system of rats: definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal CNS and in four models of parenchymal reaction.
    Glia. 7 (1): 34-40.

References for CD31 antibody

  1. Williams, K.C. et al. (1996) PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat.
    J Neurosci Res. 45 (6): 747-57.
  2. Graham, M.J. et al. (1998) In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration.
    J Pharmacol Exp Ther. 286: 447-58.
  3. MacLean, A.G. et al. (2001) Rhesus macaque brain microvessel endothelial cells behave in a manner phenotypically distinct from umbilical vein endothelial cells.
    J Neuroimmunol. 118: 223-32.
  4. Frye, C.A. & Patrick, C.W. Jr (2002) Isolation and culture of rat microvascular endothelial cells.
    In Vitro Cell Dev Biol Anim. 38 (4): 208-12.
  5. Nakao, A. et al. (2003) Carbon monoxide inhalation protects rat intestinal grafts from ischemia/reperfusion injury.
    Am J Pathol. 163: 1587-98.
  6. Seegers, H.C. et al. (2003) Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation.
    J Pharmacol Exp Ther. 306: 8-12.
  7. Haywood, L. et al. (2003) Inflammation and angiogenesis in osteoarthritis.
    Arthritis Rheum. 48: 2173-7.
  8. Wilson, E. et al. (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues.
    J Immunol Methods. 322: 137-42.
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Mouse anti Rat CD31:Biotin,clone TLD-3A12
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