- 询价
- BIO-RAD
- TZA043B
- 2025年10月17日
- E
- Human
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human IgG1 kappa
- 亚型：
Fab antibody
- 形态：
A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA043 with a biotin conjugated BiSpyCatcher version 2 (TZC002B). The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- 克隆性：
Monoclonal Antibody
- 标记物：
Biotin
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
AbD27686
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
E
- 浓度：
Antibody concentration 0.5 mg/ml
- 抗体名：
IgG1 antibody | AbD27686
- 规格：
0.1 mg
Human Anti-Human IgG1 Fc Antibody, clone AbD27686 is a recombinant monovalent Fab antibody that specifically recognizes the human IgG1 Fc region. It does not recognize other human immunoglobulin isotypes (IgG2, IgG3, IgG4, IgA, IgE, IgM) or mouse IgG2a and it has been shown to have a greatly reduced affinity for cynomologue macaque IgG (89-fold) and rhesus macaque compared to human IgG1.
AbD27686 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog (TZA043). It is also available as a bivalent Fab antibody already conjugated to biotin via coupling to the biotin conjugated BiSpyCatcher2 (TZC002B) under product code TZA043B. AbD27686 is also available without SpyTag as a purified antibody (HCA285) or conjugated to HRP (HCA285P).
This antibody has been generated by Bio-Rad using the HuCAL® antibody library and CysDisplay® technology. It is sold for research use, with implicit permission for use for the detection of therapeutic antibodies from samples obtained from experimental animals. When tested by Bio-Rad in an antigen capture pharmacokinetic assay format, this antibody performs similarly to Mouse Anti-Human IgG (CH2 Domain) Antibody clone R10Z8E9 (MCA5748G) in 10% cynomolgus macaque serum. It recognizes a different epitope on human IgG1 Fc than clone R10Z8E9.
View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies.
AbD27686 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog (TZA043). It is also available as a bivalent Fab antibody already conjugated to biotin via coupling to the biotin conjugated BiSpyCatcher2 (TZC002B) under product code TZA043B. AbD27686 is also available without SpyTag as a purified antibody (HCA285) or conjugated to HRP (HCA285P).
This antibody has been generated by Bio-Rad using the HuCAL® antibody library and CysDisplay® technology. It is sold for research use, with implicit permission for use for the detection of therapeutic antibodies from samples obtained from experimental animals. When tested by Bio-Rad in an antigen capture pharmacokinetic assay format, this antibody performs similarly to Mouse Anti-Human IgG (CH2 Domain) Antibody clone R10Z8E9 (MCA5748G) in 10% cynomolgus macaque serum. It recognizes a different epitope on human IgG1 Fc than clone R10Z8E9.
View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies.
储存信息
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
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Human anti Human IgG1 (Fc):Biotin，clone AbD27686
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