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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified mouse IgG.
- 亚型:
IgG
- 形态:
F(ab')2 fragment of IgG conjugated to biotin - liquid
- 克隆性:
Polyclonal Antibody
- 标记物:
Biotin
- 适应物种:
Mouse
- 保质期:
自发货之日起 12 个月
- 目录编号:
Polyclonal IgG
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, E, F, P
- 浓度:
F(ab')2 concentration 1.0mg/ml
- 抗体名:
IgG antibody
- 规格:
1 mg
Biotin conjugated Rabbit F(ab')2 anti Mouse IgG antibody recognizes all subclasses of mouse IgG.
Cross reactivity with rat IgG is expected. Cross reactivity to human serum proteins has been minimised by solid phase adsorbtion.
Cross reactivity with rat IgG is expected. Cross reactivity to human serum proteins has been minimised by solid phase adsorbtion.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | 1/1000 | 1/2000 |
| Flow Cytometry | ✓ | 1/50 | 1/100 |
| Immunohistology - Frozen | ✓ | 1/50 | 1/200 |
| Immunohistology - Paraffin | ✓ | 1/50 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
References for IgG antibody
- Silveira, M.R. et al. (2002) Infection with Strongyloides venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E, and hyperresponsiveness in rats.
Infect Immun. 70 (11): 6263-72. - Conlon, T.M. et al. (2012) Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells.
J Immunol. 188 (6): 2643-52. - Harper, I.G. et al. (2016) Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant.
Cell Rep. 15 (6): 1214-27. - Kushwaha, V. & Capalash, N. (2023) Evaluation of immunomodulatory potential of recombinant histidyl-tRNA synthetase (rLdHisRS) protein of Leishmania donovani as a vaccine candidate against visceral leishmaniasis.
Acta Trop. 241: 106867.
Further Reading
- Motallebzadeh, R. et al. (2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.
FASEB J. 26 (1): 51-62.
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Rabbit F(ab')2 anti Mouse IgG:Biotin
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