- 询价
- BIO-RAD
- MCA1746GA
- 2025年10月17日
- C, F, IF, IP
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Porcine CD31/human IgGFc fusion protein.
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
LCI-4
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, F, IF, IP
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
CD31 antibody | LCI-4
- 规格：
0.1 mg
Mouse anti Pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwett et al.1995). The extracellular region contains six Ig-like domains. Mouse anti Pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants (Nasu et al.1999).
Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasu et al. 1999).
Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasu et al. 1999).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/10 | 1/100 |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ | ||
| Western Blotting | × |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
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文献和实验该产品被引用文献
References for CD31 antibody
- Nasu, K. et al. (1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.
Transplantation. 68: 861-7. - Evans, P.C. et al. (2001) Signaling through CD31 protects endothelial cells from apoptosis.
Transplantation. 71 (3): 343-4. - Campos, E. et al. (2004) In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line
Vet Res. 35: 625-33. - Waksman, R. et al. (2006) Intracoronary photodynamic therapy reduces neointimal growth without suppressing re-endothelialisation in a porcine model.
Heart. 92: 1138-44. - Iohara, K. et al. (2008) A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp.
Stem Cells. 26 (9): 2408-18. - Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver.
Stem Cells. 26: 1347-55. - Tchorsh-Yutsis, D. et al. (2009) Pig embryonic pancreatic tissue as a source for transplantation in diabetes: transient treatment with anti-LFA1, anti-CD48, and FTY720 enables long-term graft maintenance in mice with only mild ongoing immunosuppression.
Diabetes. 58: 1585-94. - Gesslein, B. et al. (2010) Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion.
Mol Vis. 16: 392-407.
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54. - Rayat, G.R. et al. (2016) First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes - Chapter 3: Porcine islet product manufacturing and release testing criteria.
Xenotransplantation. 23 (1): 38-45.
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Mouse anti Pig CD31，clone LCI-4
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