- 询价
- BIO-RAD
- MCA2315GA
- 2025年10月17日
- C, F *, IP, P *, WB
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Porcine alveolar macrophages.
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
4E9/11
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, F *, IP, P *, WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
CD107a antibody | 4E9/11
- 规格：
0.1 mg
Mouse anti Pig CD107a, clone 4E9/11 recognizes porcine CD107a, a cell surface antigen, also known as lysosomal-associated membrane protein-1 or LAMP-1.
CD107a is a type 1 single pass transmembrane glycoprotein expressed on macrophages and more weakly on monocytes and granulocytes.
CD107a is a type 1 single pass transmembrane glycoprotein expressed on macrophages and more weakly on monocytes and granulocytes.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | ✓ | 1/25 | 1/50 |
| Immunohistology - Frozen | ✓ | ||
| Immunohistology - Paraffin 2 | ✓ | ||
| Immunoprecipitation | ✓ | ||
| Western Blotting | ✓ |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- Histology Positive Control Tissue
- Porcine spleen
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文献和实验该产品被引用文献
References for CD107a antibody
- Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens. 49 (4): 403-13. - Carrillo, A. et al. (2002) Isolation and characterization of immortalized porcine aortic endothelial cell lines.
Vet Immunol Immunopathol. 89 (1-2): 91-8. - Domenech, N. et al. (2003) Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.
Vet Immunol Immunopathol. 94 (1-2): 77-81. - Sánchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23. - Toka, F.N. et al. (2009) Natural killer cell dysfunction during acute infection with foot-and-mouth disease virus.
Clin Vaccine Immunol. 16: 1738-49. - Toka, F.N. et al. (2009) Activation of porcine natural killer cells and lysis of foot-and-mouth disease virus infected cells.
J Interferon Cytokine Res. 29 (3): 179-92. - Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 44: 13. - Cruz, J.L. et al. (2013) Alphacoronavirus Protein 7 Modulates Host Innate Immune Response
J Virol. 87: 9754-67.
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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Mouse anti Pig CD107a，clone 4E9/11
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