- 询价
- BIO-RAD
- MCA1757T
- 2025年10月17日
- C, E, F *, P
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human myeloperoxidase
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
2C7
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, E, F *, P
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
Myeloperoxidase antibody | 2C7
- 规格：
20 µg/0.2 mg
| 规格： | 20 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 0.2 mg | 产品价格： | 询价 |
Mouse anti Human myeloperoxidase antibody, clone 2C7 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond.
Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.
Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003).
Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.
Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | ||
| Flow Cytometry 1 | ✓ | 1/50 | 1/100 |
| Immunohistology - Frozen | ✓ | 1/1000 | 1/5000 |
| Immunohistology - Paraffin | ✓ | 1/500 | 1/1000 |
| Western Blotting 2 | ✓ |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2Mouse anti Human myeloperoxidase antibody, clone 2C7 recognises myeloperoxidase under non-reducing conditions (Audrian et al. 1997).
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human bone marrow
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文献和实验该产品被引用文献
Source Reference
- Audrain, M.A. et al. (1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.
Clin Exp Immunol. 107 (1): 127-34.
References for Myeloperoxidase antibody
- Patry, Y.C. et al. (2003) Difference in antigenic determinant profiles between human and rat myeloperoxidase.
Clin Exp Immunol. 132 (3): 505-8. - Sloane, A.J. et al. (2005) Proteomic analysis of sputum from adults and children with cystic fibrosis and from control subjects.
Am J Respir Crit Care Med. 172: 1416-26. - Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71. - Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71. - Luo, B. et al. (2013) Immunopathology features of chronic rhinosinusitis in high-altitude dwelling Tibetans.
Allergy Rhinol (Providence). 4: e69-76. - Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
Vet Immunol Immunopathol. 159 (1-2): 91-6. - Behnen, M. et al. (2014) Immobilized immune complexes induce neutrophil extracellular trap release by human neutrophil granulocytes via FcγRIIIB and Mac-1.
J Immunol. 193 (4): 1954-65. - Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
Vet Comp Oncol. 14 (4): 409-16.
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Mouse anti Human Myeloperoxidase，clone 2C7
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