- 询价
- BIO-RAD
- MCA43R
- 2025年10月17日
- C *, F, IF
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Rat thymocyte membrane glycoproteins.
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Rat
- 保质期：
自发货之日起 12 个月
- 目录编号：
OX-1
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C *, F, IF
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
CD45 antibody | OX-1
- 规格：
0.25 mg/1 mg/0.1 mg
| 规格： | 0.25 mg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | 询价 |
| 规格： | 0.1 mg | 产品价格： | 询价 |
Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa.
Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
This product is routinely tested in flow cytometry on rat splenocytes
Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
This product is routinely tested in flow cytometry on rat splenocytes
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/100 | |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen 1 | ✓ | ||
| Immunohistology - Paraffin | × |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of * fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
References for CD45 antibody
- Standring, R. et al. (1978) The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.
Eur J Immunol. 8 (12): 832-9. - Sunderland, C.A. et al. (1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
Eur J Immunol. 9 (2): 155-9. - Woollett, G.R. et al. (1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
Eur J Immunol. 15 (2): 168-73. - Martín, A. et al. (1995) Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis.
Clin Exp Immunol. 99 (2): 283-8. - Giezeman-Smits, K.M. et al. (1999) The regulatory role of CD45 on rat NK cells in target cell lysis.
J Immunol. 163 (1): 71-6. - Murakami, K. et al. (2000) Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase.
Int Immunol. 12 (2): 169-76. - Ermert, L. et al. (2001) Comparison of different detection methods in quantitative microdensitometry.
Am J Pathol. 158: 407-17. - Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
Invest Ophthalmol Vis Sci. 42: 170-6.
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Mouse anti Rat CD45，clone OX-1
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