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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Normal human peripheral blood lymphocytes.
- 亚型:
IgG1
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Human
- 保质期:
自发货之日起 12 个月
- 目录编号:
LT8
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, F, IP
- 浓度:
IgG concentration 1.0mg/ml
- 抗体名:
CD8 antibody | LT8
- 规格:
0.1 mg/25 µg/0.2 mg
| 规格: | 0.1 mg | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 25 µg | 产品价格: | 询价 |
| 规格: | 0.2 mg | 产品价格: | 询价 |
Mouse anti Human CD8 antibody, clone LT8 recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells.
The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.
The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/50 | 1/100 |
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or cells or 100μl human whole blood
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文献和实验该产品被引用文献
References for CD8 antibody
- Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
J Immunol. 167 (8): 4593-9. - Manninen, A. & Saksela, K. (2002) HIV-1 Nef interacts with inositol trisphosphate receptor to activate calcium signaling in T cells.
J Exp Med. 195 (8): 1023-32. - Parnes, J.R. (1989) Molecular biology and function of CD4 and CD8.
Adv Immunol. 44: 265-311. - Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
J Histochem Cytochem. 57: 1159-67. - Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. 12: 2. - Nelson, M. et al. (2010) Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus).
J Med Microbiol. 59: 1107-13. - Gibbings, D.J. et al. (2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.
BMC Immunol. 8: 12. - Junker, A. et al. (2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions.
Brain. 130: 2789-99.
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文献支持
Mouse anti Human CD8,clone LT8
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