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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2020R |
| 英文名称 | phospho-KLF5 (Ser275) Rabbit pAb |
| 中文名称 | 磷酸化KLF5抗体 |
| 英文别名 | KLF5(phospho Ser275); KLF5(phospho S275); Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5; Transcription factor BTEB 2; Transcription factor BTEB2; KLF5_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human KLF5 around the phosphorylation site of Ser275 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Oncoproteins > Cell survival & death Cardiovascular > Heart > Apoptosis Cardiovascular > Heart > Hypertrophy > Transcription factors Cell Biology > Apoptosis > Intracellular > p53 Pathway Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Proto-oncogenes Epigenetics and Nuclear Signaling > Transcription > Domain Families > Krueppel like factor Epigenetics and Nuclear Signaling > Transcription > Krueppel like factor Stem Cells > Mesenchymal Stem Cells > Intracellular |
| 亚基 | Interacts with WWP1. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed only in testis and placenta. |
| 翻译后修饰 | Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. |
| 相似性 | Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. |
| 功能 | Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Lane 1: Human HeLa cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human HT-1080 cell lysates
Lane 4: Human A549 cell lysates
Lane 5: Human A431 cell lysates
Primary: Anti-phospho-KLF5 (Ser275) (bs-2020R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 60 kDa
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文献和实验[IF={{ 1.664 }}] {Li W et al. Association of the invasiveness of colon cancer with the expression of C/EBPα. Oncol Lett. 2019 Feb;17(2):1974-1979.} {WB} {Human}
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