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- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-8235R |
| 英文名称 | FRMD4A Rabbit pAb |
| 中文名称 | FRMD4A蛋白抗体 |
| 英文别名 | FERM domain containing 4A; FERM domain-containing protein 4A; FRM4A_HUMAN; FRMD4; Frmd4a. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Dog, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FRMD4A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 115 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Other Antibodies |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 相似性 | Contains 1 FERM domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | FRMD4A is a 1,039 amino acid protein that localizes to cytoplasm and contains one FERM domain. The gene encoding FRMD4A maps to human chromosome 10p13. Spanning nearly 135 million base pairs, chromosome 10 makes up approximately 4.5% of total DNA in cells and encodes nearly 1,200 genes. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FRMD4A) Polyclonal Antibody, Unconjugated (bs-8235R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti- FRMD4A (bs-8235R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
K562(Human) Cell Lysate at 30 ug
Primary: Anti- FRMD4A (bs-8235R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
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文献和实验该产品被引用文献
[IF={{ 40.8 }}] {Jiang Zedong. et al. Revealing the crucial roles of suppressive immune microenvironment in cardiac myxoma progression. SIGNAL TRANSDUCT TAR. 2024 Aug;9(1):1-16} {IF} {Human}
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FRMD4A Rabbit pAb(bs-8235R)-50ul/100ul/200ul
¥1180 - 2800





