HIF-1 Alpha Rabbit pAb(bs-20398R)-50ul/100ul/200ul
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HIF-1 Alpha Rabbit pAb(bs-2039

8R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-20398R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号 bs-20398R
    英文名称 HIF-1 Alpha Rabbit pAb
    中文名称 缺氧诱导因子1α/HIF-1α 抗体
    英文别名 ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit(basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor-1a; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8.
    产品应用 Flow-Cyt=1μg/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.
    交叉反应 Human (Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep)
    抗体来源 Rabbit
    免疫原 KLH conjugated synthetic peptide derived from human HIF-1 Alpha
    亚型 IgG
    性状 Liquid
    纯化方法 affinity purified by Protein A
    克隆类型 Polyclonal
    理论分子量 92 kDa
    浓度 1mg/ml
    储存液 Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
    研究领域

    Cancer > Invasion/microenvironment > Hypoxia > HIF

    Cancer > Invasion/microenvironment > Hypoxia > Hydroxylases

    Epigenetics and Nuclear Signaling > Cardiovascular/Immune > Hypoxia > HIF

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Jumonji containing proteins

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Methylation
    亚基 Interacts with COPS5 subunit of COP9 signalosome complex,leading to the regulation of its stability. Interacts with TSGA10(By similarity). Efficient DNA binding requires heterodimerizationof an alpha and a beta/ARNT subunit. Binds to the TAZ-type 1domains of CREBBP and EP300. Interacts with NCOA1, NCOA2, APEX andHSP90. Interacts with VHL which docks HFA1 to the E3 ubiquitinligase complex for subsequent destruction. Interaction, via the ODDdomain, with the beta domain of VHLL, protects HIF1A fromdestruction by competing against the destructive targetinginitiated by VHL.
    亚细胞定位 Cytoplasm. Nucleus.
    组织特异性 Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
    翻译后修饰 In normoxia, is hydroxylated on Pro-402 and Pro-564 in theoxygen-dependent degradation domain (ODD) by EGLN1/PHD1 andEGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564.The hydroxylated prolines promote interaction with VHL, initiatingrapid ubiquitination and subsequent proteasomal degradation. Underhypoxia, proline hydroxylation is impaired and ubiquitination isattenuated, resulting in stabilization.
    In normoxia, is hydroxylated on Asn-803 by HIF1AN, thusabrogating interaction with CREBBP and EP300 and preventingtranscriptional activation.
    S-nitrosylation of Cys-800 may be responsible for increasedrecruitment of p300 coactivator necessary for transcriptionalactivity of HIF-1 complex.
    Acetylation of Lys-532 by ARD1 increases interaction with VHLand stimulates subsequent proteasomal degradation.
    Requires phosphorylation for DNA-binding.
    相似性 Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
    功能 Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.
    保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料     缺氧诱导因子1Alpha不仅对于机体在缺氧条件下维持正常的生理功能具有特别重要的意义,并在肿瘤的生长以及神经细胞凋亡等病理过程中起重要作用. HIF1 alpha能调节许多下游基因的表达水平.     哺乳动物细胞在低氧压力条件下出现HIF。HIF是一种转录因子，对细胞的缺氧起稳定作用。

     

    应用 推荐稀释比例
    {Flow-Cyt} {1μg/Test}

     

    Sample: Lane 1: Recombinant human HIF-1 Alpha protein, N-Trx-His(bs-42326P) Primary: Anti-HIF-1 Alpha (bs-20398R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kDa Observed band size: 47 kDa
    HIF-1 Alpha Rabbit pAb(bs-2039
    The Hela (treated with 500uM CoCl2 for 6 hours) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-HIF-1 Alpha antibody (bs-20398R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 9.4 }}] {Xia Lei. et al.pH-responsive photothermal effect and heterojunction formation for tumor-specific pyroelectrodynamic and nanozyme-catalyzed starvation therapy..Acta Biomaterialia.2025 Mar 18:S1742-7061(25)00204-1.} {IHC,IF} {Mouse}

    [IF={{ 8.2 }}] {Shaorong Li. et al. Dual-functional 3D-printed porous bioactive scaffold enhanced bone repair by promoting osteogenesis and angiogenesis. MATER TODAY BIO. 2024 Feb;24:100943} {IHC} {Rabbit}

    [IF={{ 6.304 }}] {Yan Liu. et al. Long noncoding RNA LINC00518 induces radioresistance by regulating glycolysis through an miR-33a-3p/HIF-1α negative feedback loop in melanoma. Cell Death Dis. 2021 Mar;12(3):1-19} {WB,IP} {Human}

    [IF={{ 6 }}] {Xijin Zhu. et al. Feedback regulation between AMPK and HIF-1α contributes to color stability via energy metabolism and mitochondrial efficiency regulation of yak meat. LWT-FOOD SCI TECHNOL. 2024 Apr;:116031} {WB} {Bovine}

    [IF={{ 5.742 }}] {Pan Cao. et al. Sirtuin1 attenuates acute liver failure by reducing reactive oxygen species via hypoxia inducible factor 1α. WORLD J GASTROENTERO. 2022 May 7; 28(17): 1798–1813} {WB} {Human,Mouse}

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    HIF-1 Alpha Rabbit pAb(bs-20398R)-50ul/100ul/200ul
    ￥1180 - 2800