Sample:
Lane 1: Lymph node (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: Testis (Rat) Lysate at 40 ug
Lane 4: Cerebellum (Mouse) Lysate at 40 ug
Lane 5: K562 (Human) Cell Lysate at 30 ug
Lane 6: Hela (Human) Cell Lysate at 30 ug
Lane 7: MDA-MB-231 (Human) Cell Lysate at 30 ug
Lane 8: U251 (Human) Cell Lysate at 30 ug
Lane 9: U937 (Human) Cell Lysate at 30 ug
Lane 10: A673 (Human) Cell Lysate at 30 ug
Primary: Anti-PRKAR2A (bs-55176R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
- ￥1180 - 1980
- Bioss
- bs-55176R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 产品编号 | bs-55176R |
| 英文名称 | PRKAR2A Rabbit pAb |
| 中文名称 | 蛋白激酶A调节亚基2α抗体 |
| 英文别名 | KAP2; KAP2_HUMAN; MGC3606; PKR 2; PKR2; PRKA R2; PRKAR 2; PRKAR2; Protein kinase A RII alpha subunit; Protein kinase cAMP dependent regulatory type II alpha; cAMP dependent protein kinase regulatory subunit alpha 2; cAMP dependent protein kinase regulatory subunit RII alpha; cAMP dependent protein kinase type II alpha regulatory chain; cAMP dependent protein kinase type II alpha regulatory subunit; cAMP-dependent protein kinase type II-alpha regulatory subunit. |
| 产品应用 | WB=1:500-2000, ICC/IF=1:50 - 1:200, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | Recombinant human PRKAR2A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 49 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy Metabolism > Types of disease > Cancer Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > PKA Signal Transduction > Second Messenger > Nucleotide Messenger > cAMP |
| 亚基 | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. |
| 亚细胞定位 | Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and the cell membrane. |
| 组织特异性 | Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. |
| 翻译后修饰 | Phosphorylated by the activated catalytic chain. |
| 相似性 | Belongs to the cAMP-dependent kinase regulatory chain family. |
| 功能 | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus, where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. Mouse Serine 96 (designated Ser 99 in human) is a phosphorylation site on the PKA II?regulatory subunit. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:50 - 1:200} |
| {ELISA} | {1:5000-10000} |
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PRKAR2A Rabbit pAb(bs-55176R)-50ul/100ul
￥1180 - 1980
