VDAC1 Recombinant Rabbit mAb, Mitochondrial Loading Control(bsm-52251R)-50ul/100ul/25ul
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VDAC1 Recombinant Rabbit mAb,

Mitochondrial Loading Control(bsm-52251R)-50ul/100ul/25ul
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  • ￥800 - 2500
  • Bioss已认证
  • bsm-52251R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/25ul

    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    规格：25ul产品价格：￥800.0
    产品编号bsm-52251R
    英文名称VDAC1 Recombinant Rabbit mAb, Mitochondrial Loading Control
    中文名称等电压依赖性阴离子通道（内参）重组兔单抗
    英文别名voltage-dependent anion channel; hVDAC1; MGC111064; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31 HL; Porin 31HL; Porin 31HM; VDAC 1; VDAC; VDAC1; Voltage dependent anion channel 1; Voltage dependent anion selective channel protein 1; VTA1_HUMAN.
    产品应用WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human VDAC1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量32 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Cellular metabolic process

    Cancer > Cell Death > Apoptosis > Metabolism

    Cancer > Cell Death > Apoptosis > Mitochondrial

    Cell Biology > Apoptosis > Mitochondrial

    Isotype/Loading Controls > Loading Controls > VDAC1 - Mitochondrial Preps

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism

    Metabolism > Pathways and Processes > Metabolism processes > Apoptosis

    Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers

    Metabolism > Types of disease > Cancer

    Signal Transduction > Metabolism > Energy Metabolism

    Signal Transduction > Metabolism > Mitochondrial

    Signal Transduction > Metabolism > Plasma Membrane > Channels

    Tags & Cell Markers > Subcellular Markers > Organelles > Mitochondria

    亚基Interacts with VPS4B. Interacts with CHMP1B. Interacts with CHMP2A; the interaction probably involves the open conformation of (polymerized) CHMP2A. May interact with CHMP3. Interacts with CHMP5; the interaction involves soluble CHMP5. Interacts with IST1.
    亚细胞定位Cytoplasm. Endosome membrane; Peripheral membrane protein (Probable).
    相似性Belongs to the VTA1 family.
    功能Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding.
    保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Voltage dependent anion selective channel protein 1 (VDAC/Porin) belongs to the eukaryotic mitochondrial porin family and forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel allows diffusion of small hydrophilic molecules; it adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation selective. VDAC/Porin expression is observed in the heart,liver and skeletal muscle.

     

    应用推荐稀释比例
    {WB}{1:500-5000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {ICC/IF}{1:50-200}

     

    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    P‌‌araformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VDAC1 Monoclonal Antibody, Unconjugated(bsm-52251R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    VDAC1 Recombinant Rabbit mAb,
    25 ug total protein per lane of various lysates (see on figure) probed with VDAC1 monoclonal antibody, unconjugated (bsm-52251R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    VDAC1 Recombinant Rabbit mAb,
    4% P‌‌araformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (VDAC1) monoclonal Antibody, unconjugated (bsm-52251R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

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    该产品被引用文献

    [IF={{ 4.213 }}] {Yang Jiao. et al. Lysine demethylation KDM5B downregulates SIRT3-mediated mitochondrial glucose and lipid metabolism in diabetic neuropathy. DIABETIC MED. 2022 Sep;:e14964} {WB} {Mouse}

    [IF={{ 2.9 }}] {Songfeng Chen. et al. Upregulation of mitochondrial PGK1 by ROS-TBC1D15 pathway promotes neuronal death after oxygen-glucose deprivation/reoxygenation injury. BRAIN RES. 2024 Feb;1825:148724} {WB} {Mouse}

    [IF={{ 1.7 }}] {Jinhua Wang. et al.Indirubin induces apoptosis in ovarian cancer cells via the mitochondrial pathway.AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH.2024 Oct 15;16(10):6119-6129.} {Western blot} {Human}

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    文献支持
    VDAC1 Recombinant Rabbit mAb, Mitochondrial Loading Control(bsm-52251R)-50ul/100ul/25ul
    ￥800 - 2500