ACE2 Recombinant Rabbit mAb(bsm-52614R)-50ul/100ul/25ul
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ACE2 Recombinant Rabbit mAb(bs

m-52614R)-50ul/100ul/25ul
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  • ￥800 - 2500
  • Bioss已认证
  • bsm-52614R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/25ul

    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    规格：25ul产品价格：￥800.0
    产品编号bsm-52614R
    英文名称ACE2 Recombinant Rabbit mAb
    中文名称血管紧张素转换酶重组兔单抗
    英文别名ACE-2; ACE 2; Angiotensin converting enzyme 2; ACE related carboxypeptidase; ACEH; Angiotensin converting enzyme homolog; Angiotensin converting enzyme like protein; Angiotensin I Converting Enzyme(peptidyl dipeptidase A) 2; Angiotensin I converting enzyme 2; DKFZP434A014; EC 3.4.17; angiotensin-converting enzyme 2 precursor; ACE2_HUMAN; Angiotensin-converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15; Processed angiotensin-converting enzyme 2.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human ACE2
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量92 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Blood > Blood Pressure regulation

    Cardiovascular > Heart > Contractility > Inotropics

    Cardiovascular > Vasculature > Vasoconstriction

    Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Metalloprotease > ACEs

    Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus

    亚基

    Homodimer (PubMed:32132184).
    Interacts with the catalytically active form of TMPRSS2 (PubMed:21068237).
    Interacts with SLC6A19; this interaction is essential for expression and function of SLC6A19 in intestine (By similarity).
    Interacts with ITGA5:ITGB1 (PubMed:15276642, PubMed:33102950).
    Probably interacts (via endocytic sorting signal motif) with AP2M1; the interaction is inhibited by phosphorylation of Tyr-781 (PubMed:33436498).
    Interacts (via PDZ-binding motif) with NHERF1 (via PDZ domains); the interaction may enhance ACE2 membrane residence (PubMed:34189428).
    (Microbial infection) Interacts with SARS coronavirus/SARS-CoV spike protein.
    (Microbial infection) Interacts with SARS coronavirus-2/SARS-CoV-2 spike protein.
    (Microbial infection) Interacts with human coronavirus NL63 spike protein.
    (Microbial infection) Interacts with human coronavirus NL63/HCoV-NL63 spike glycoprotein.

    亚细胞定位

    Processed angiotensin-converting enzyme 2: Secreted. Cell membrane; Single-pass type I membrane protein.

    组织特异性

    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level) (PubMed:15141377).
    Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level) (PubMed:15141377).
    Expressed in the renal proximal tubule and the small intestine (at protein level) (PubMed:18424768).
    Expressed in heart, kidney, testis, and gastrointestinal system (at protein level) (PubMed:10969042, PubMed:10924499, PubMed:15231706, PubMed:12459472, PubMed:15671045, PubMed:32715618, PubMed:32170560).
    In lung, expressed at low levels in some alveolar type 2 cells, the expression seems to be individual-specific (at protein level) (PubMed:32425701, PubMed:15141377, PubMed:32715618, PubMed:32170560, PubMed:33432184).
    Expressed in nasal epithelial cells (at protein level) (PubMed:33432184, PubMed:32333915).
    Coexpressed with TMPRSS2 within some lung alveolar type 2 cells, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells (PubMed:32413319, PubMed:32327758, PubMed:32358202).
    Coexpressed with TMPRSS4 within mature enterocytes (PubMed:32404436).

    翻译后修饰

    N-glycosylation on Asn-90 may limit SARS infectivity.
    Proteolytic cleavage by ADAM17 generates a secreted form.
    Belongs to the peptidase M2 family.


    相似性

    Belongs to the peptidase M2 family.

    功能

    Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402).
    Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186).
    Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627).
    Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165).
    In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582).
    (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63.

    保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:50-200}
    {IF}{1:100-500}

     

    ACE2 Recombinant Rabbit mAb(bs
    P‌‌araformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACE2) Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    ACE2 Recombinant Rabbit mAb(bs
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACE2) Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    ACE2 Recombinant Rabbit mAb(bs
    (Negative control) P‌‌araformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACE2 Monoclonal Antibody, Unconjugated(bsm-52614R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    ACE2 Recombinant Rabbit mAb(bs
    P‌‌araformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACE2 Monoclonal Antibody, Unconjugated(bsm-52614R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    ACE2 Recombinant Rabbit mAb(bs
    P‌‌araformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACE2 Monoclonal Antibody, Unconjugated(bsm-52614R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    ACE2 Recombinant Rabbit mAb(bs
    P‌‌araformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACE2) Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining
    ACE2 Recombinant Rabbit mAb(bs
    25 ug total protein per lane of various lysates (see on figure) probed with ACE2 monoclonal antibody, unconjugated (bsm-52614R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    ACE2 Recombinant Rabbit mAb(bs
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACE2 Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
    ACE2 Recombinant Rabbit mAb(bs
    P‌‌araformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ACE2 Monoclonal Antibody, Unconjugated (bsm-52614R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 5.776 }}] {Endika Prieto-Fernández. et al. Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate. Emerg Microbes Infec. 2021;10(1):1065-1076} {WB} {Human}

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    ACE2 Recombinant Rabbit mAb(bsm-52614R)-50ul/100ul/25ul
    ￥800 - 2500