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- abinScience
- China
- VK072042
- 2025年10月16日
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- 保存条件：
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- 保质期：
1 year
- 英文名：
Recombinant MERS-CoV nsp4 Protein, N-His
- 供应商：
abinScience
- 规格：
50ug/100ug/1mg
| Product name | Recombinant MERS-CoV nsp4 Protein, N-His |
|---|---|
| Catalog No. | VK072042 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Target | / |
| Expression system | E. coli |
| Accession | K9N638 |
| Protein length | Val3151-Gln3247 |
| Alternative Names | Replicase polyprotein 1a, pp1a, ORF1a polyprotein, Host translation inhibitor nsp1, nsp1, Leader protein, Non-structural protein 2, nsp2, p65 homolog, Papain-like protease nsp3, PL-PRO, EC:3.4.19.12, EC:3.4.22.-, Non-structural protein 3, nsp3, PL2-PRO, Non-structural protein 4, nsp4, 3C-like proteinase nsp5, 3CL-PRO, 3CLp, EC:3.4.22.69, nsp5, Non-structural protein 6, nsp6, Non-structural protein 7, nsp7, Non-structural protein 8, nsp8, RNA-capping enzyme subunit nsp9, Non-structural protein 9, nsp9, EC:2.7.7.50, Non-structural protein 10, nsp10, Growth factor-like peptide, GFL, Non-structural protein 11, nsp11 |
| Predicted molecular weight | 12.96 kDa |
| Applications.1 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Protein length.1 | Val3151-Gln3247 |
| Species | Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV) |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003, abinScience has inherited advanced technologies and high-quality standards. In particular, the XtenCHO™ system developed by ProteoGenix, which has two modes of transient expression and stable expression, has been widely recognized as a core technology to improve the efficiency of protein production, providing a solid foundation for ab...
Product categories and application fields.
Main products.
10000+ high-activity proteins.
15000+ antibody series products:
Classic monoclonal antibody.
Monoclonal antibody
Internal control antibody, labeled antibody.
High-quality phosphorylation antibodies.
Highly specific nanobodies and small molecule antibodies.
Functional antibodies in vivo.
Low-background flow cytometry antibodies.
DNA/RNA antibodies and PEG antibodies.
1000+ reagent kit series products.
Large-scale packaging customization services: We offer personalized antibody design, protein production, molecular optimization, and experimental development services.
Application field
Infectious disease research: including viruses, superbugs, parasites, etc.
Neuroscience research: Supporting research on neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
Immunology research: Covers the research needs of immune activation, inhibition, and allergies and abnormal reactions.
Oncology research: Supporting the development of tumor biology and anti-tumor drugs.
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文献和实验• Low phosphorus promotes NSP1-NSP2 heterodimerization to enhance strigolactone biosynthesis and regulate shoot and root architecture in rice., PMID:37794682
• Nucleoporin Nsp1 surveils the phase state of FG-Nups., PMID:39356635
• Coronavirus Nsp1: Immune Response Suppression and Protein Expression Inhibition., PMID:34659188
• Mechanisms of Coronavirus Nsp1-Mediated Control of Host and Viral Gene Expression., PMID:33540583
• Nsp1 of SARS-CoV-2 stimulates host translation termination., PMID:34793288
• Chikungunya virus nsP1 induces migrasome formation., PMID:35933040
• The art of hijacking: how Nsp1 impacts host gene expression during coronaviral infections., PMID:38385526
• Rhesus rotavirus NSP1 mediates extra-intestinal infection and is a contributing factor for biliary obstruction., PMID:39348381
• Drug targeting Nsp1-ribosomal complex shows antiviral activity against SARS-CoV-2., PMID:35323109
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
, and usually final protein yield between 0.1 and 2 mg/g cells is acceptable. Another concern is protein degradation. Especially with Pichia , protease activity during cell lysis and purification is always an issue. The importance of N-terminal degradation
The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins
of interest, but some affinity tails are able to be linked to either the N- or C-terminus of the protein of interest. The choice of affinity tail to use for the expression of any particular protein is empirical since the factors leading to the high expression of recombinant
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