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- 2025年10月16日
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| 产品编号 | bs-17680P |
| 英文名称 | phospho-SF1 (Ser82) Antibody Blocking Peptide |
| 中文名称 | 磷酸化剪接因子1封闭多肽 |
| 英文别名 | splicing factor 1 (phospho S82); p-splicing factor 1 (phospho S82); BBP; D11S636; Mammalian branch point-binding protein mBBP; OTTHUMP00000069749; OTTHUMP00000069750; OTTHUMP00000069752; splicing factor 1; Transcription factor ZFM1; ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; ZNF162. |
| 纯化方法 | HPLC |
| 亚细胞定位 | Nuclear |
| 组织特异性 | Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart. |
| 翻译后修饰 | Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463. |
| 相似性 | Belongs to the BBP/SF1 family.
Contains 1 CCHC-type zinc finger. Contains 1 KH domain. |
| 功能 | SF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
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文献和实验Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
or from the literature are missing. In this article, processing parameters for DNA, peptide, antibody, and carbohydrate microarrays are outlined. The applicability of the model experiments is demonstrated and described in detail on the example of short oligonucleotides.
Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage
a significant level of antibodies specific to the nonphosphorylated peptide is present in the antisera, an enhancement step is used to obtain a useful phospho-specific antibody. Although these enhanced antisera are suitable for many applications
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