- ￥4880 - 15800
- Bioss
- bs-41516P
- 2025年10月16日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 规格：
100ug/500ug
| 规格： | 100ug | 产品价格： | ￥4880.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥15800.0 |
| 产品编号 | bs-41516P |
| 英文名称 | Recombinant SARS-Cov-2 Spike RBD protein, His |
| 中文名称 | 重组SARS冠状病毒2 Spike蛋白RBD |
| 英文别名 | SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2; Recombinant SARS-Cov-2 Spike RBD protein, His |
| 中文别名 | 新冠病毒S蛋白RBD; 新冠S蛋白RBD; SARS-CoV-2 S蛋白RBD; |
| 性状 | Liquid |
| 纯化方法 | AC |
| 理论分子量 | 24.7 |
| 实际分子量 | 30-35 kDa |
| 浓度 | ＞0.5 mg/ml |
| 储存液 | 0.2μm filtered solution of PBS (pH7.4) with 150mM NaCl. |
| 研究领域 | Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus |
| 亚基 | Spike glycoprotein: Homotrimer; each monomer consists of a S1 and a S2 subunit (PubMed:32155444, PubMed:32075877). The resulting peplomers protrude from the virus surface as spikes (By similarity). Interacts with the accessory proteins 3a and 7a. Spike protein S1: Binds to human ACE2. |
| 亚细胞定位 | Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ; Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion. |
| 组织特异性 | The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes. Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins (PubMed:32155444). Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor (By similarity). The presence of a furin polybasic cleavage site sets SARS-CoV-2 S apart from SARS-CoV S that possesses a monobasic S1/S2 cleavage site processed upon entry of target cells (PubMed:32155444). Highly decorated by heterogeneous N-linked glycans protruding from the trimer surface. |
| 翻译后修饰 | The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes. Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins (PubMed:32155444). Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor (By similarity). The presence of a furin polybasic cleavage site sets SARS-CoV-2 S apart from SARS-CoV S that possesses a monobasic S1/S2 cleavage site processed upon entry of target cells (PubMed:32155444). Highly decorated by heterogeneous N-linked glycans protruding from the trimer surface. |
| 功能 | attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
5位院士为代表，超12篇CNS大作，5月新冠研究汇总——全方位围攻新冠病毒，值得收藏
的鸡尾酒疗法也引起了大家的关注，不同的抗体可以识别结合域上不同的抗原表位，在未来的临床应用中能够避免免疫逃逸的理想单克隆抗体对。 5 月 8 日，军事医学科学院生物工程研究所陈薇院士团队和西湖大学周强团队强强联合，在预印本平台 bioRxiv 上线题为：A potent neutralizing human antibody reveals the N-terminal domain of the Spike protein of SARS-CoV-2 as a site
技术资料暂无技术资料 索取技术资料
Recombinant SARS-Cov-2 Spike RBD protein, His(bs-41516P)-100ug/500ug
￥4880 - 15800
