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- Bioss
- bs-0312P-PE
- 2025年10月16日
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100ul
| 产品编号 | bs-0312P-PE |
| 英文名称 | Avidin, PE conjugated |
| 中文名称 | 荧光素PE标记亲合素 |
| 英文别名 | Avidin / PE |
| 性状 | Liquid |
| 浓度 | 1.0 mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Stored at 2-8℃ for one year. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer), each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity. In its tetrameric form, avidin is estimated to be between 66–69 kDa in size.Ten percent of the molecular weight is attributed to carbohydrate content composed of four to five mannose and three N-acetylglucosamine residues. The carbohydrate moieties of avidin contain at least three unique oligosaccharide structural types that are similar in structure and composition. |
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
This method, described in 1981 by Hsu and associates, makes fundamental use of the covalent and irreversible binding seen between avidin, an egg white protein, and biotin, a vitamin (1 ). By establishing a biotin link, through avidin
或溶液的振子强度为 f kj =[8 π 2 m ／（ 3he 2 ） ]V kj |P kj | 2 。能与它相比较的实验值 f exp ，可从实测 （ v ） dv 求得。但 v 是以 cm -1 为单位的振动频率（亦即波数）。在结晶的场合下，因分子的排列并不杂乱， f kj 中的 P kj 对各分子可改为（ pE ） kj 。这里的 pE 是表示 P 在振动的电场方向的分量。还有，对于 f exp 也可进行同样的改写。
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