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100ul
| 产品编号 | bs-0294P-Cy7 |
| 英文名称 | Goat IgG / Cy7 |
| 中文名称 | Cy7标记羊IgG(同型对照) |
| 英文别名 | Goat Immunoglobulin G/Cy7 |
| 中文别名 | 荧光素Cy7标记羊IgG(细胞流式同型对照) |
| 性状 | Liquid |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
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文献和实验Early development of primary motor neurons and somites in Zebrafish Embryos
boundaries. The ZNP-1 and F6 treated embryos will be treated with a secondary antibody, Fluorescent Goat anti-mouse IgG, so that the stained neurons and somites can be observed under a fluorescent microscope. Procedure: 1. Dechorionate 24
的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光,可以检测FL1-FL4四个通道/4个颜色。 不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的,比如Calibur的FL3(第3通道)能检测PE-Texas Red, PE-Cy5, PerCP, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-Texas Red, PE-Cy5, PerCP。 Calibur第3通道能检测的PerCP-Cy
, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-TexasRed, PE-Cy5, PerCP。Calibur第3通道能检测的PerCP-Cy5.5在FACSAria上是第4通道检测。Calibur和LSR都能检测4个颜色,但是第4个通道检测荧光素是不一样的 搭配荧光素原则 搭配流式荧光素有2个原则:每个通道只能选择1种荧光素;各个通道之间的荧光素可以随意搭配,但需注意 1、每个通道只能选择1种荧光素。以Calibur为例说明,Calibur
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