Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-CD71 antibody (bs-0988R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
- ￥2980
- Bioss
- bs-0988R-FITC
- 2025年10月15日
- 产品信息以Bioss网站为准
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100ul
| 产品编号 | bs-0988R-FITC |
| 英文名称 | TFRC Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的转铁蛋白受体（CD71）抗体 |
| 英文别名 | Transferrin receptor protein 1; Transferrin receptor; CD71; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; Transferrin receptor(p90 CD71); TRFR; TFR1_HUMAN. |
| 产品应用 | Flow-Cyt=1μg /Test, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Transferrin receptor |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cardiovascular > Blood > Serum Proteins Immunology > Cell Type Markers > CD > Non-lineage Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Stem Cells > Hematopoietic Progenitors > Lymphoid > B Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Erythrocytic Lineage Stem Cells > Neural Stem Cells > Surface Molecules |
| 亚基 | Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with Machupo arenavirus GPC. |
| 亚细胞定位 | Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 翻译后修饰 | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. |
| 相似性 | Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. |
| 功能 | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg /Test} |
| {IF} | {1:100-500} |
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Fluorochrome Absorption and Emission Spectra
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Fluorochrome Absorption and Emission
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TFRC Rabbit pAb, FITC conjugated(bs-0988R-FITC)-100ul
￥2980
