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100T/48S
| 产品编号 | AK237 |
| 英文名称 | Lipoprotein lipase Assay Kit |
| 中文名称 | 脂蛋白酯酶(LPL)检测试剂盒 |
| 英文别名 | Lipoprotein lipase (LPL) Assay Kit; LPL Assay Kit; |
| 保存条件 | 详见说明书. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | 意义:脂蛋白酯酶 (Lipoprotein lipase, LPL) 是脂肪细胞、心肌细胞、骨骼肌细胞、乳腺细胞及巨噬细胞等实质细胞合成的 一种酶,可催化甘油三脂水解为脂肪酸和单酸甘油酯,以供组织氧化供能和贮存,并在不同的组织表现出不同的生理意义。原理:脂蛋白酯酶水解 4-硝基苯棕榈酸酯产生 4-硝基,在 400nm 有特征吸收峰。 |
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文献和实验Lysosomal Acid Lipase: Assay and Purification
Lysosomal acid lipase (LAL, E.C. 3.1.1.13) is a hydrolase involved in the intracellular degradation of lipoproteins. LAL was first described in fibroblasts (1 ), and since then found in all analyzed tissues with the exception of erythrocytes
Bianchi, E., Steinkuhler, C., Taliani, M., Urbani, A., Francesco, R.D., and Pessi, A. 1996. Synthetic depsipeptide substrates for the assay of human hepatitis C virus protease. Anal. Biochem. 237:239‐244
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODS
CBQCA Protein Quantitation Kit (pdf) 10 ng/mL - 150 µg/mL Functions well in the presence of lipids and detergents (to determine the protein content of lipoprotein samples or lipid�protein mixtures)
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