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500ug
| 产品编号 | bs-17352P |
| 英文名称 | Homer (1b+1c) Antibody Blocking Peptide |
| 中文名称 | 神经细胞早期基因封闭多肽 |
| 英文别名 | Homer 1; HOMER; Homer homolog 1; Homer protein homolog 1; Homer1; HOMER1B; HOMER1C; Neuronal immediate early gene; SYN47; Ves 1. HOME1_HUMAN |
| 纯化方法 | HPLC |
| 研究领域 | Neuroscience > Cell Type Marker > Neuron marker > Synapse marker Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Glutamate Receptors |
| 亚基 | Interacts with GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2,SHANK1 and SHANK3. Interacts with IFT57 and OPHN1. Isoform 1 encodes a coiled-coil structure that mediates homo- and heteromultimerization. |
| 亚细胞定位 | Cytoplasmic; Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Postsynaptic density of neuronal cells. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. |
| 相似性 | Belongs to the Homer family. Contains 1 WH1 domain. |
| 功能 | Homer is a neuronal immediate early gene that is enriched at excitatory synapses and selectively binds metabotropic glutamate receptors. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq, Jul 2008] |
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文献和实验Purification Of Anti Peptide Antibody
the antibody can be concentrated by sweating the dialysis bags, or by spin-concentrating. Bring the azide concentration up to 0.1 and store at 4 deg C for up to three months. For longer storage freeze in aliquots and store at -80 degC or add glycerol
Blocking of unwanted non-specific staining
receptors). Crossreactivity of the secondary reagents with endogenous proteins. Blocking of endogenous enzymes Endogenous enzymes such as AlkPhos, AcPhos and esterases are destroyed by boiling Ref, even a short time at 100 C. Peroxidase
Purification Of Anti Peptide Antibody
Purification Of Anti Peptide Antibody Claire Walczak January 1996 Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls
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