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- bs-14494P
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500ug
| 产品编号 | bs-14494P |
| 英文名称 | EBNA 3C Antibody Blocking Peptide |
| 中文名称 | EB病毒核抗原-3C封闭多肽 |
| 英文别名 | EBNA3C; EBNA 4B; EBNA 6; EBNA 3C; EBNA6_EBVB9; EBV; EBV nuclear antigen 6; Epstein Barr nuclear antigen 6; HHV4; Human Herpesvirus 4. |
| 纯化方法 | HPLC |
| 研究领域 | Microbiology > Organism > Virus > DNA Virus > double stranded DNA Virus > EBNA |
| 亚基 | Interacts with host CTPB1; this interaction seems mportant for EBNA6-mediated transcriptional repression. Interacts ith host MYC; this interaction enhances MYC stability. Interacts via N-terminus) with host RBPJ. |
| 亚细胞定位 | Host nucleus matrix. Note=Associated with he nuclear matrix. |
| 相似性 | Belongs to the herpesviridae EBNA-6 family. |
| 功能 | Plays an essential role for the activation and mmortalization of human B-cells. Represses transcription of viral romoters TP1 and Cp through interaction with host RBPJ, and nhibits EBNA2-mediated activation of these promoters. Since Cp is he promoter for all EBNA mRNAs, EBNA6 probably contributes to a egative autoregulatory control loop. Alternatively, EBNA6 also egulates the transcription of the EBV oncogene LMP1 in a cell ycle-dependent manner. It modulates the activity of several host roteins involved in cell cycle regulation including host cyclin A, YC, RB and p27 mainly through binding to the host SCF(SKP2) omplex. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | EBNA 3C is a latent viral nuclear protein expressed in EBV transformed lymphoblastic cell lines and is essential for EBV mediated transformation of these cell lines. This viral nuclear protein is essential for EBV mediated positive immunoblastic lymphomas, and functions as a transcription factor. |
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文献和实验Purification Of Anti Peptide Antibody
the antibody can be concentrated by sweating the dialysis bags, or by spin-concentrating. Bring the azide concentration up to 0.1 and store at 4 deg C for up to three months. For longer storage freeze in aliquots and store at -80 degC or add glycerol
Blocking of unwanted non-specific staining
receptors). Crossreactivity of the secondary reagents with endogenous proteins. Blocking of endogenous enzymes Endogenous enzymes such as AlkPhos, AcPhos and esterases are destroyed by boiling Ref, even a short time at 100 C. Peroxidase
Purification Of Anti Peptide Antibody
Purification Of Anti Peptide Antibody Claire Walczak January 1996 Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls
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