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RT
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见标签
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- 供应商:
鹿森生物
- 规格:
100MG

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文献和实验Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Blue Native Gel Electrophoresis
at 4°C 1xCathode+dye 0.01% Serva G in 1xCathode buffer It takes a while to dissolve ServaG. Store at 4°C BN-sample buffer Serva G 50 mg 2 x BisTrisACA 500 ul 75% Sucrose 400 ul H2O 100 ul Store at -20°C Solubilization buffer
Purification of DNA from lambda phage
5OmM Tris,ph 8,20 141 TES (100 mM Tris pH 8,100 mM EDTA,1 % SDS).Add 20 pLI of 10 mg/ml proteinase K and incubate one hour (can be left overnight)at 37℃. D.Add 1 vol of buffered phenol.Mix well and microcentrifuge 2 min.Transfer aqueous layer to new
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