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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human IGLC1 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
Ig Lambda Light Chain
- 抗体英文名:
HRP-conjugated Ig Lambda Light Chain Polyclonal antibody
- 抗体名:
HRP-conjugated Ig Lambda Light Chain Polyclonal antibody
- 规格:
100ul
经过测试的应用
| Positive WB detected in | human plasma |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-20758 targets Ig Lambda Light Chain in WB applications and shows reactivity with human samples.
| 经测试应用 | WB |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag14693 Product name: Recombinant human IGLC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC007782 Sequence: MAWTVLLLGLLSHCTGSGTSYVLTQPASVSVAPGQTARITCGGSNLGSKSVNWYQLRPGQAPILVVYENKERPAGIPERLSALTSEETATLTISSVVAGDEADYFCQVWDTTSQQYVFGTGTQVTVLGQPKANPTVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADGSPVKAGVETTKPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin lambda constant 1 (Mcg marker) |
| 别名 | Human IgG Lambda Light Chain, Human IgG lambda chain, IgG Lambda Light Chain, IGLC1, IGLC |
| 计算分子量 | 233 aa, 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC007782 |
| 基因名称 | IgG Lambda Light Chain |
| Gene ID (NCBI) | 3537 |
| RRID | AB_2920508 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody detects the lambda light chain of human immunoglobulin.
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文献和实验| Species | Application | Title |
|---|---|---|
| WB | Neurochem Res Necrostatin-1 Against Sevoflurane-Induced Cognitive Dysfunction Involves Activation of BDNF/TrkB Pathway and Inhibition of Necroptosis in Aged Rats.Authors - Chunping Yin | |
| WB | Curr Med Sci USP19 Stabilizes TAK1 to Regulate High Glucose/Free Fatty Acid-induced Dysfunction in HK-2 CellsAuthors - Xiao-Hui Yan |
affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse IgG1 heavy chain do stain rat IgG2a and IgG2b . Procedure: First stain HRP Developing solution Second stain AP
20. 7bis- block endogenous peroxidase by incubating in 0.1%NaN 3 and 0.3% H 2 O 2 for 30 min. Wash thrice. 8- add the HRP-conjugated avidin (50 to 100 µl, dilution 1:300 -500 in nTBS-Tween
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