- ￥3200
- Bioss
- IHC0333
- 2025年10月15日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 规格：
50T
| 产品编号 | IHC0333 |
| 英文名称 | Glutamine synthetase Ready-To-Use IHC Kit |
| 中文名称 | 谷氨酰胺合成酶/谷氨酸氨连接酶即用型免疫组化试剂盒 |
| 英文别名 | GLNS; GLUL; GS; PIG43; PIG59; Glutamate ammonia ligase; Glutamate decarboxylase; Glutamine synthetase |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 亚基 | Decamer; composed of two pentamers (PubMed:18005987). Interacts with PALMD (By similarity). Interacts with RHOJ (PubMed:30158707). Interacts with BEST2; this interaction tethers a fraction of GLUL to the membrane, causing a decrease of cytosolic glutamine synthase (GS) activity and inhibits the chloride channel activity of BEST2 by affecting the gating at the aperture in the absence of intracellular glutamate (PubMed:36289327). |
| 亚细胞定位 | #Cell membrane #Cytoplasm #Endoplasmic reticulum #Membrane #Microsome #Mitochondrion |
| 组织特异性 | Expressed in endothelial cells. |
| 翻译后修饰 | Acetylated by EP300/p300; acetylation is stimulated by increased glutamine levels and promotes ubiquitin-mediated proteasomal degradation. Palmitoylated; undergoes autopalmitoylation. Ubiquitinated by ZNRF1 (By similarity). Ubiquitinated by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(CRBN), leading to proteasomal degradation (PubMed:26990986). |
| 相似性 | Belongs to the glutamine synthetase family. |
| 功能 | Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed:16267323, PubMed:30158707, PubMed:36289327). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed:18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed:30158707). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed:26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed:36289327). |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 背景资料 | Glutamine synthase is part of the glutamine synthetase family. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor. It also has an important role in controlling metabolic regulations of neurotransmitter glutamate. Because of the multiple functions and importance of GS in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of GS is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. |
| 应用 | 推荐稀释比例 |
Immunohistochemical analysis of paraffin embedded rat kidney tissue slide using IHC0333 (Glutamine synthetase Kit).
Immunohistochemical analysis of paraffin embedded human liver tissue slide using IHC0333 (Glutamine synthetase Kit).
Immunohistochemical analysis of paraffin embedded mouse liver tissue slide using IHC0333 (Glutamine synthetase Kit).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Glutamine synthetase Ready-To-Use IHC Kit(IHC0333)-50T
￥3200
