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| 产品编号 | IHC0114 |
| 英文名称 | Alpha smooth muscle Actin Ready-To-Use IHC Kit |
| 中文名称 | 肌动蛋白α/α-SMA/α Actin单克隆即用型免疫组化试剂盒 |
| 英文别名 | alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Rat, Mouse, Human |
| 研究领域 |
Cardiovascular > Cardiovascular Markers > Cell Markers > Smooth Muscle Cells Cardiovascular > Heart > Contractility > Contractile Proteins > Actins Cardiovascular > Vasculature > Smooth muscle cell (SMC) > Cytoskeleton Cardiovascular > Vasculature > Vasculature Markers > Arterial Developmental Biology > Lineage specification > Mesoderm Signal Transduction > Antibodies > alpha smooth muscle Actin Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Stem Cells > Lineage Markers > Mesoderm Stem Cells > Mesenchymal Stem Cells > Myogenesis |
| 亚基 | Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 翻译后修饰 | Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced (By similarity). |
| 相似性 | Belongs to the actin family. |
| 功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. alpha-Actin expression is limited to various types of muscle, whereas beta- and gamma-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| 应用 | 推荐稀释比例 |
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文献和实验Use of Monoclonal Antibodies in Immunohistochemistry
differentiation of as many as 75% of poorly differentiated or undifferentiated tumors (1 ). The rapid advancement of IHC has also been fueled by the commercial availability of hundreds of MAbs and detection systems, often provided in kit form containing ready
Protein purification: skeletal muscle myosin
Overview SKELETAL MUSCLE MYOSIN A two-headed hexomeric actin-activated ATPase consisting of two heavy chains of ~200 kDa and four light chains of ~20kDa. The tail portion
Use of Internaland External Standardsor Reference RNAs for A
to being an ideal internal control under the broadest range of experimental conditions, see below). It is therefore necessary to identify the appropriate control RNA for the particular set of experimental RNA samples to be studied. ß-actin ß-actin mRNA
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