- ￥4760 - 7650
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- D60111
- 2025年10月17日
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5mg/10mg
| 规格： | 5mg | 产品价格： | ￥4760.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥7650.0 |
| 产品编号 | D60111 |
| 英文名称 | Hoechst 33258 analog 2 |
| 理论分子量 | 422.5248 |
| 保存条件 | -20℃ |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Hoechst 33258 analog 2 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution |
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文献和实验Chemistry of Minor Groove Binder–Oligonucleotide Conjugates
Singh, M.P., Joseph, T., Kumar, S., Bathini, Y., and Lown, J.W. 1992. Synthesis and sequence‐specific DNA binding of a topoisomerase inhibitory analog of Hoechst 33258 designed for altered base and sequence recognition. Chem. Res. Toxicol. 5:597
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation&
instrument can be accomplished using FITC- and PE-conjugated antibodies and the far red DNA binding dyes 7-aminoactinomycin D or LDS-751 . Hoechst 33342 and 33258 or DAPI can be used on dual laser (488 nm/UV) instruments for simultaneous analysis
Simultaneous analysis of DNA content and surface
- and PE-conjugated antibodies and the far red DNA binding dyes 7-aminoactinomycin D or LDS-751 . Hoechst 33342 and 33258 or DAPI can be used on dual laser (488 nm/UV) instruments for simultaneous analysis with two or three fluorochrome-conjugated
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