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1mg/1mL x 10mM (in DMSO)
| 规格: | 1mg | 产品价格: | ¥451.0 |
|---|---|---|---|
| 规格: | 1mL x 10mM (in DMSO) | 产品价格: | ¥1207.0 |
| 产品编号 | D56914 |
| 英文名称 | TC HSD 21 |
| 理论分子量 | 422.32 |
| 保存条件 | Powder: -20℃ for 3 years | In solvent: -80℃ for 1 year |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | TC HSD 21 is a potent and highly selective inhibitor of 17β-hydroxysteroid dehydrogenase type 3 (IC50 = 14 nM). |
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文献和实验minutes. 5. Add media, M15 to inactivate the trypsin. About 2 mls to 1 x 6-cm dish or 4 - 5 mls to 1 x 10-cm dish. 6. With a transfer pipette, pipet up and down several times to separate the cells and break any colonies. 7. Determine
,so just grow the cells at 30 or 37 ℃ when making BL21 competent cells.However,it has been suggested that the efficiency of BL21 prepared using Inoue method may be improved by treating it with DTT before freezing (add to 3.5% v/v of a 2.2M DTT,10mM KAc pH
7、Spin at 2500 x g (5000 rpm in a Sorvall GSA,5500 rpm in a Sorvall SS-34,or 3000 rpm in a Beckman J-6B centrifuge)for 10 min.at 4℃.4度2500g离心10分钟。 8、Resuspend Cell s gently in 20 ml of ice cold "TB".小心用20ml预冷TB重悬细胞。 9、Add DMSO to a final
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