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1mg/1mL x 10mM (in DMSO)
| 规格: | 1mg | 产品价格: | ¥3127.0 |
|---|---|---|---|
| 规格: | 1mL x 10mM (in DMSO) | 产品价格: | ¥9397.0 |
| 产品编号 | D56564 |
| 英文名称 | SERCA2a activator 1 |
| 理论分子量 | 551.66 |
| 保存条件 | Powder: -20℃ for 3 years | In solvent: -80℃ for 1 year |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SERCA2a activator 1 is a sarco/endoplasmic reticulum Ca2+-dependent ATPase 2a activator. SERCA2a activator 1 decreases phospholamban inhibition and enhances the systolic and diastolic functions of the heart. |
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文献和实验.N. Cabili, C. Trapnell, L. Goff, M. Koziol, B. Tazon-Vega, A. Regev, J.L. Rinn, Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses, Genes Dev 25(18) (2011) 1915-27. [2] G.D. Yang, X.Z. Lu
PCR amplification of variable regions (SOE-Protocol)
DMSO 4 to 4.5 µl PCR 10x buffer 3 µl MgCl2, 25 mM 0,3 µl Taq-polymerase (5U/µl) ad 50 µl Water VH amplification from G2a/2b first strand 5-10 µl first-strand reaction (with COH 32 primer) 2 µl VH front primer (10 pmol/µl stock) 2 µl VH
bacteriophage and plasmid DNA. Nature468(7320):67–71. doi: 10.1038/nature09523 19. Cong L, Ran FA, Cox D, Lin S, Barretto R,Habib N, Hsu PD, Wu X, Jiang W, Marraffi niLA, Zhang F (2013) Multiplex genome engineeringusing CRISPR/Cas systems. Science339(6121
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