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- 技术资料
- 规格:
1mL x 10mM (in DMSO)/500mg
| 规格: | 1mL x 10mM (in DMSO) | 产品价格: | ¥184.0 |
|---|---|---|---|
| 规格: | 500mg | 产品价格: | ¥231.0 |
| 产品编号 | D54775 |
| 英文名称 | 1-Methylindazole-3-carboxylic acid |
| 中文名称 | 1-甲基-3-吲唑甲酸 |
| 理论分子量 | 176.172 |
| 保存条件 | Powder: -20℃ for 3 years | In solvent: -80℃ for 1 year |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | 1-Methylindazole-3-carboxylic acid is an excellent solvent with a proper boiling point. |
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文献和实验Clonality - X Chromosome Inactivation Assay
(Life Technologies) 10X PCR buffer (100 mM Tris-HCL pH 8.3, 500 mM KCL, 15 mM MgCl2, 0,01 % w/v gelatin, autoclaved) (Perkin Elmer) dNTP, each 10 mM (Perkin Elmer) 7-deaza dGTP, 10 mM (Boehringer Mannheim) DMSO, minimum 99.5%, GC (Sigma
Analysis of Proteins using Small Format 2D Gel Electrophoresis
Ltd.)) for 5 minutes on ice. The nuclei are removed by centrifugation at 11,500 x g for 5 minutes and the supernatant stored at -20C for later analysis by gel electrophoresis. The incorporation of radioactive methionine into the proteins is determined by TCA
刚开始做,希望多认识几个朋友,做了好几次,都没有出来结果,请多指点Gel Recipe6.6 g Urea1.59 ml Acrylamide/Bis (28.38g:1.62g to 100ml)2.4 ml 10% Triton X-1000.48 ml ampholyte pH5-80.12 ml Ampholyte pH3-10 (optional)2.4 ml Water12 μl TEMED90 μl 3% Ammonium PersulfateThis
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