- ￥1350 - 3000
- Proteintech
- 67915-1-Ig
- 2025年10月13日
- WB, IHC, FC (Intra), ELISA
- Mouse
- human, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human DDX3 protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, FC (Intra), ELISA
- 靶点：
DDX3
- 抗体英文名：
DDX3 Monoclonal antibody
- 抗体名：
DDX3 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | LNCaP cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67915-1-Ig targets DDX3 in WB, IHC, IF, FC (Intra), CoIP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, rat, hamster |
| 免疫原 |
CatNo: Ag29972 Product name: Recombinant human DDX3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 312-662 aa of BC011819 Sequence: DLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked |
| 别名 | DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X |
| 计算分子量 | 73 kDa |
| 观测分子量 | 71-73 kDa |
| GenBank蛋白编号 | BC011819 |
| 基因名称 | DDX3 |
| Gene ID (NCBI) | 1654 |
| RRID | AB_2918669 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O00571 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX3X, also named DBX, DDX3, and HLP2, belongs to the DEAD-box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73kd is the target band. 60-65kd is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Sci Adv PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activitiesAuthors - Nina Đukić |
| rat | IF | CNS Neurosci Ther A novel stroke rehabilitation strategy and underlying stress granule regulations through inhibition of NLRP3 inflammasome activationAuthors - Qingzhu Wang |
| human | WB | Int Immunopharmacol Bortezomib induces cell apoptosis and increases the efficacy of αPD-1 in BCR::ABL T315I mutation CML by targeting UBE2Q1Authors - Fengyu Jiang |
| human,hamster | WB,CoIP | Adv Sci (Weinh) DDX3 Regulates the Cap-Independent Translation of the Japanese Encephalitis Virus via Its Interactions with PABP1 and the Untranslated Regions of the Viral GenomeAuthors - Chenxi Li
|
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