- ￥1350 - 3000
- Proteintech
- 17720-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CSK protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
CSK
- 抗体英文名：
CSK Polyclonal antibody
- 抗体名：
CSK Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Raji cells, K-562 cells, HL-60 cells, Jurkat cells, rat lung tissue |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human lung tissue, human skin tissue, human placenta tissue, human kidney tissue, human brain tissue, human ovary tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17720-1-AP targets CSK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12103 Product name: Recombinant human CSK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 83-450 aa of BC106073 Sequence: FHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | c-src tyrosine kinase |
| 别名 | C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK |
| 计算分子量 | 450 aa, 50 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC106073 |
| 基因名称 | CSK |
| Gene ID (NCBI) | 1445 |
| RRID | AB_2084150 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41240 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IHC | Cancer Res miR-31 Displays Subtype Specificity in Lung Cancer.Authors - Mackenzie L Davenport |
| human | WB | Cell Chem Biol A strategy to assess the cellular activity of E3 ligase components against neo-substrates using electrophilic probes.Authors - Benika J Pinch |
| human | WB | J Immunol Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells.Authors - Hagberg Niklas N |
| mouse | WB,IF | bioRxiv Crk mediates Csk-Hippo signaling independently of Yap tyrosine phosphorylation to induce cell extrusionAuthors - Abdul Hannan |
| human | IP | Neoplasia SUMOylation of Csk Negatively Modulates its Tumor Suppressor Function.Authors - Nan Cui |
upon treatment of macrophages with TLR ligands, such as double-stranded RNA [e.g., poly-r(I:C)] (which binds to TLR3), Gram-negative bacterial lipopolysaccharides (LPSs) (which bind to TLR4), the synthetic lipoprotein Pam3CSK4 (Pam3CysSerLys4; InvivoGen, San
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
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