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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, ELISA
- 靶点:
KRAS-4B-specific
- 抗体英文名:
KRAS-4B-specific Polyclonal antibody
- 抗体名:
KRAS-4B-specific Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, mouse brain tissue, Recombinant protein |
| Positive IHC detected in | rat brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16155-1-AP targets KRAS-4B-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| 别名 | KRAS-4B, KRAS, pan RAS, C K RAS, c Ki ras |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | NM_004985 |
| 基因名称 | KRAS |
| Gene ID (NCBI) | 3845 |
| RRID | AB_2134119 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01116 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAS gene family encodes very similar 21KD proteins and are homologs of rodent sarcoma virus genes that have transforming abilities. KRAS has two isoforms, 4A and 4B. This antibody is specific for KRAS-4B, which is also named as KRAS-2B.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | Nat Biotechnol Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors.Authors - Christopher W Davies | |
| human | Nat Biomed Eng Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.Authors - Mahmoud Labib | |
| human | WB | Cancer Res Targeting eIF4A-Dependent Translation of KRAS Signaling Molecules.Authors - Kamini Singh |
| human | WB | Cell Chem Biol Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.Authors - Sascha Röth |
| human | WB | Sci Signal Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.Authors - Andrew M Waters |
| human | WB | Life Sci Alliance A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95Authors - Kristen M Rehl |
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