- ￥1350 - 3000
- Proteintech
- 15140-1-AP
- 2025年10月13日
- WB, IP, IHC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human GLO1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IP, IHC, ELISA
- 靶点：
GLO1
- 抗体英文名：
GLO1 Polyclonal antibody
- 抗体名：
GLO1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Raji cells, HeLa cells, rat liver tissue, HepG2 cells, mouse liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15140-1-AP targets GLO1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7314 Product name: Recombinant human GLO1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-184 aa of BC001741 Sequence: MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glyoxalase I |
| 别名 | Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21-25 kDa |
| GenBank蛋白编号 | BC001741 |
| 基因名称 | GLO1 |
| Gene ID (NCBI) | 2739 |
| RRID | AB_2109890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q04760 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer. The molecular mass of the dimer was 46 kDa determined by gel permeation chromatography.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Adv Sci (Weinh) Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast CancerAuthors - Jindong Xie |
| human | WB | Brain C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.Authors - Scott P Allen |
| mouse | WB | Front Pharmacol Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders miceAuthors - Jin-Bo Xie |
| mouse | WB,IF | Phytomedicine Protective effects of Huang-Lian-Jie-Du Decoction on diabetic nephropathy through regulating AGEs/RAGE/Akt/Nrf2 pathway and metabolic profiling in db/db mice.Authors - Dan Tang |
| human | WB | Oncotarget Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells.Authors - Shoma Tamori
|
| mouse | WB | Life Sci Alliance Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.Authors - Shengya Tian |
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