- ￥1350 - 3000
- Proteintech
- 55462-1-AP
- 2025年10月13日
- WB, IHC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, FC (Intra), IP, ELISA
- 靶点：
Myosin Light Chain 2/MLC-2V
- 抗体英文名：
Myosin Light Chain 2/MLC-2V Polyclonal antibody
- 抗体名：
Myosin Light Chain 2/MLC-2V Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | rat heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myosin, light chain 2, regulatory, cardiac, slow |
| 别名 | MLC 2v, MYL2, MLC 2, MLC2, MLC-2 |
| 计算分子量 | 19 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | NM_000432 |
| 基因名称 | Myosin Light Chain 2 |
| Gene ID (NCBI) | 4633 |
| RRID | AB_2881342 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10916 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Cell Mol Immunol Sexual dimorphism of lung immune-regulatory units imprint biased pulmonary fibrosisAuthors - Peng Xiang |
| mouse | IF | Stem Cell Reports Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.Authors - Kiwon Ban |
| human | WB | iScience SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury.Authors - Anteneh Getachew |
| human | WB | Int J Mol Med Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis.Authors - Chao Ci |
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