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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human KI67 protein
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
IHC, Cytometric bead array, Indirect ELISA
- 靶点:
Ki-67
- 抗体英文名:
Ki-67 Monoclonal antibody, PBS Only (Capture)
- 抗体名:
Ki-67 Monoclonal antibody, PBS Only (Capture)
- 规格:
100ug
产品信息
66555-5-PBS targets Ki-67 as part of a matched antibody pair:
MP50237-3: 66555-5-PBS capture and 66555-6-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | IHC, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26266 Product name: Recombinant human KI67 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1201-1300 aa of NM_002417 Sequence: AGTLPGSKRQLQTPKEKAQALEDLAGFKELFQTPGHTEELVAAGKTTKIPCDSPQSDPVDTPTSTKQRPKRSIRKADVEGELLACRNLMPSAGKAMHTPK |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | antigen identified by monoclonal antibody Ki-67 |
| 别名 | KI67, MKI67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Ki 67 |
| 计算分子量 | 359 kDa |
| GenBank蛋白编号 | NM_002417 |
| 基因名称 | KI67 |
| Gene ID (NCBI) | 4288 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P46013 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验Immuno-Laser Capture Microdissection
for the proliferation-associated antigen Ki67, using MIB1 antibody (dilution, 1:10) in conjunction with the Quickstain kit. Magnification x100. Fig. F : Prostate immunostained for cytokeratin. Stromal tissue has been removed by LCM, leaving
a. 制备活性较好的细胞悬液,调节浓度至107个/ml。 b. 在小试管(5×50mm)中加入100ul细胞悬液,再加入100ul待检McAb的样品,混匀,4℃作用30-90分钟。 c. 用洗涤液(PBS 900ml,小牛血清50ml,4%NaN3 50ml)洗二次,每次加洗涤液1-5ml,1000r/min离心5分钟,弃上清。加入100ul荧光抗体,4℃作用30-90分钟。 d. 同法洗涤,将细胞重新悬于20-30ul含10%甘油和10-100ug苯二胺/ml
再加入10μl(含1mCi) 125 I ↓ 加入10μl氯胺-T(5mg/ml溶于0.05M 磷酸盐缓冲液),孵育时间不超过30秒 ↓ 加入100μl偏重亚硫酸钠(1.2mg/ml溶于 0.05M磷酸缓冲液) ↓ 加入200μl KI溶液(少许结晶KI溶于0.05M
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