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100ul
| 产品编号 | bs-19542R-APC |
| 英文名称 | NUP133 Rabbit pAb, APC conjugated |
| 中文名称 | APC标记的核孔复合蛋白Nup133抗体 |
| 英文别名 | 133 kDa nucleoporin; FLJ10814; hNUP 133; hNUP133; MGC21133; NU133_HUMAN; Nuclear pore complex protein Nup133; Nucleoporin 133kDa; Nucleoporin NUP133; NUP 133; NUP133; OTTHUMP00000061095. |
| 产品应用 | ICC/IF=1:50-200, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NUP133 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Nuclear Pore Complex Signal Transduction > Protein Trafficking > Nuclear Import / Export |
| 亚细胞定位 | Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores. |
| 组织特异性 | Involved in poly(A)+ RNA transport. |
| 相似性 | Belongs to the nucleoporin Nup133 family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-500} |
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文献和实验Fluorochrome Absorption and Emission Spectra
light at 488 nm for three-color flow cytometric analysis (Fig. 1). BD Biosciences Pharmingen also offers monoclonal antibodies and proteins conjugated to allophycocyanin (APC) and avidin conjugated to Texas Red™. Both of these dyes
Fluorochrome Absorption and Emission
for three-color flow cytometric analysis (Fig. 1). BD Biosciences Pharmingen also offers monoclonal antibodies and proteins conjugated to allophycocyanin (APC) and avidin conjugated to Texas Red™. Both of these dyes are useful in experiments
区、1个IgC样区、1个跨膜疏水区和1个由30个氨基酸组成的胞内区。不同于其他B7家族分子的是，它具有负向调节免疫应答的作用。PD-1和PD-L1是一对共抑制分子[3]。 这对共抑制分子，对癌细胞是如何发挥作用的呢。 憋急，听我慢慢道来！ 人类身体健康的卫士「T细胞」的活化是需要接收「双重信号」的：首先，T细胞表面受体（TCR）识别由抗原提呈细胞（APCs）提呈的抗原肽，使T细胞初步活化；然后，APC表面的共刺激分子与T细胞表面的相应共刺激分子结合，使T
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