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- bs-14620R-BF680
- 2025年09月30日
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100ul
| 产品编号 | bs-14620R-BF680 |
| 英文名称 | ERF3B Rabbit pAb, BF680 conjugated |
| 中文名称 | BF680标记的ERF3B蛋白抗体 |
| 英文别名 | eRF3b; ERF3B_HUMAN; Eukaryotic peptide chain release factor GTP-binding subunit ERF3B; Eukaryotic peptide chain release factor subunit 3b; G1 to S phase transition 2; G1 to S phase transition protein 2 homolog; Gspt2; GST2; peptide chain release factor 3. |
| 产品应用 | ICC/IF=1:50-200, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ERF3B |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Interacts with ETF1 (By similarity). Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with UPF1 and PABPC1. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Highly expressed in IUCC stage II colorectal cancer (CRC). |
| 相似性 | Belongs to the GTP-binding elongation factor family. ERF3 subfamily. |
| 功能 | Involved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | This gene encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.[provided by RefSeq, Mar 2010] |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-500} |
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文献和实验In Situ Immunofluorescence Analysis: Immunofluorescence Microscopy
cells (A) or after removal of cytoskeletal component (B) or in nuclear matrix preparations (C) . Run μ2 proteins were detected with a rabbit polyclonal Run μ2 antibody and an fluorescein isothiocyanate-conjugated antirabbit secondary antibody. DAPI
. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
Antigen Design Sera Purification Tech Sheet
. The carrier protein contains many epitopes that stimulate T-helper cells, which help induce the B-cell response. Many different carrier proteins can be used for coupling to synthetic peptides. The most commonly selected carriers are keyhole limpet hemacyanin
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