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- bs-13020R-PacificBlue
- 2025年09月30日
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100ul
| 产品编号 | bs-13020R-PacificBlue |
| 英文名称 | DNA Polymerase lambda Rabbit pAb, Pacific Blue conjugated |
| 中文名称 | Pacific Blue标记的DNA聚合酶λ/DNA pol λ抗体 |
| 英文别名 | BETA N; BETAN; DNA directed DNA polymerase lambda; DNA polymerase beta 2; DNA polymerase beta-2; DNA polymerase kappa; DNA polymerase kappa DNA polymerase beta N; DNA polymerase lambda; DNA polymerase lamda2; DPOLL_HUMAN; EC 2.7.7.7, EC 4.2.99.; FLJ46002; OTTHUMP00000020321; OTTHUMP00000020323; OTTHUMP00000059179; Pol beta2; POL KAPPA; Pol Lambda; POLKAPPA; POLL; Polymerase DNA directed lambda. |
| 产品应用 | IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human DNA Polymerase lambda |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Binds PCNA. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in a number of tissues. Abundant in testis. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| 相似性 | Belongs to the DNA polymerase type-X family. _x000D_ Contains 1 BRCT domain. |
| 功能 | Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | DNA polymerase lambda (pol lambda), also designated DNA polymerase lambda or Pol Beta2, is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. Encoded by the POLL gene, pol lambda is a member of the DNA polymerase type-X family. Pol lambda extends primer-terminal mispairs opposite nondamaged DNA templates, suggesting that it may assist in extending mismatched base pairs during normal DNA replication. In addition, pol ?may play a role in the mutagenic bypass of T-T dimers. Proliferating cell nuclear antigen (PCNA), a protein essential to DNA replication, interacts with pol lambda and thus influences the ability of pol ?to synthesize DNA. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-500} |
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文献和实验Studies of the Ubiquitin Proteasome System
Biomedicals Life Science Division, or see recipe ) or HRP‐conjugated anti–rabbit Ig and chemiluminescent detection reagents (SuperSignal West Pico, Pierce, or ECL Plus, GE Healthcare) Biomax MR film (Kodak
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
Ballard, J.L., Peeva, V.K., deSilva, C.J., Lynch, J.L., and Swanson, N.R., 2007. Comparison of Alexa Fluor and CyDye for practical DNA microarray use. Mol. Biotechnol. 36:175‐183.
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
Taq DNA polymerase a 5 u/µl 1 U 0.2 µl
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