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- Bioss
- bs-12790R-Bio
- 2025年09月30日
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100ul
| 产品编号 | bs-12790R-Bio |
| 英文名称 | ZP2 Rabbit pAb, Biotin conjugated |
| 中文名称 | Biotin标记的卵透明带糖蛋白2抗体 |
| 英文别名 | OTTHUMP00000115849; Processed zona pellucida sperm-binding protein 2; Zona pellucida glycoprotein 2; zona pellucida glycoprotein ZP2; Zona pellucida protein A; Zp-2; ZP2; ZP2_HUMAN; ZPA. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, ELISA=1:500-5000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ZP2 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Reproduction > Fertilisation |
| 亚基 | Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein._x000D_ Processed zona pellucida sperm-binding protein 2: Secreted, extracellular space, extracellular matrix. Note=The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida. |
| 组织特异性 | Oocytes. |
| 翻译后修饰 | Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. _x000D_ Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos (By similarity). Proteolytically cleaved in the N-terminal part probably by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. _x000D_ -glycosylated; N-linked glycans are of high-mannose/hybrid type, as well as bi-, tri- and tetra-antennary sialylated complex types. _x000D_ O-glycosylated; contains sulfate-substituted glycans. |
| 相似性 | Belongs to the ZP domain family._x000D_
ZPA subfamily._x000D_ Contains 1 ZP domain. |
| 功能 | The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-500} |
| {ELISA} | {1:500-5000} |
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ZP2 Rabbit pAb, Biotin conjugated(bs-12790R-Bio)-100ul
￥2980
