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- bs-5754R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-5754R-HRP |
| 英文名称 | SP17 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的精子表面蛋白Sp17抗体 |
| 英文别名 | Band 34; Cancer/testis antigen 22; CT22; SP17 1; Sp17-1; SP17_HUMAN; SPA17; Sperm autoantigenic protein 17; Sperm protein 17; Sperm surface protein Sp17. |
| 产品应用 | WB=1000-10000, IHC-P=1:100-500, IHC-F=1:100-500, ELISA=1:500-5000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SP17/CT22 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Kinases/phosphatases Cell Biology > Cell Cycle > Kinases/Phosphatases Immunology > Immune System Diseases > Autoimmune Signal Transduction > Second Messenger > Nucleotide Messenger > cAMP |
| 亚基 | Homodimer (By similarity). May interact with ROPN1. |
| 亚细胞定位 | Membrane; Peripheral membrane protein (Potential). |
| 组织特异性 | Testis and sperm specific. |
| 相似性 | Contains 1 IQ domain. |
| 功能 | Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | SP17 is a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.[provided by RefSeq, Jan 2009]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ELISA} | {1:500-5000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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