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- bs-13733R-Cy3
- 2025年09月30日
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100ul
| 产品编号 | bs-13733R-Cy3 |
| 英文名称 | GAL4 Rabbit pAb, Cy3 conjugated |
| 中文名称 | Cy3标记的调节蛋白GAL4抗体 |
| 英文别名 | Gal4p; GAL81; Homo sapiens galectin4 mRNA complete cds; Regulatory protein GAL4; GAL4_YEAST. |
| 产品应用 | ICC/IF=1:50-200, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae GAL4 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Other factors Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Gap Junctions Signal Transduction > Metabolism > Energy Metabolism Tags & Cell Markers > Fusion / Marker Proteins > GAL4 |
| 亚基 | Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. |
| 亚细胞定位 | Nuclear |
| 翻译后修饰 | Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. |
| 相似性 | Contains 1 Zn(2)-C6 fungal-type DNA-binding domain. |
| 功能 | This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. As the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation, this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (amino acids 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. Fields and coworkers have taken advantage of these findings by the development of a unique “two-hybrid” system using GAL4 fusions in yeast to identify specific protein-protein interactions. |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-500} |
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文献和实验Construction and Use of Glycan Microarrays
) Printing buffer (see recipe ) Controls: unmodified BSA and HSA (negative controls) and BSA conjugated with Cy3 or Alexa Fluor 647
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