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GLUT1 Rabbit pAb, FITC conjuga

ted(bs-0472R-FITC)-100ul
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  • ￥2980
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  • bs-0472R-FITC
  • 2025年10月15日
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      100ul

    产品编号bs-0472R-FITC
    英文名称GLUT1 Rabbit pAb, FITC conjugated
    中文名称FITC标记的葡萄糖转运蛋白1抗体
    英文别名Glucose Transporter GLUT1; GT-1; GLUT-1; GLUT 1; Solute carrier family 2; facilitated glucose transporter member 1; Glucose transporter type 1; erythrocyte/brain; DYT17; DYT18; Erythrocyte/brain HepG2 glucose transporter; Erythrocyte/hepatoma glucose transporter; Glucose transporter 1; Glucose transporter type 1; Glucose transporter type 1 erythrocyte/brain; Glucose transporter type 1, erythrocyte/brain; GLUT; GLUT1; GLUT1DS; GLUTB; GT1; GTG1; Gtg3; GTR1_HUMAN; HepG2 glucose transporter; MGC141895; MGC141896; PED; RATGTG1; SLC2A 1; SLC2A1; Solute carrier family 2(facilitated glucose transporter), member 1; Solute carrier family 2 facilitated glucose transporter member 1.
    产品应用Flow-Cyt=1μg/Test, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human GLUT1
    亚型IgG
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates

    Cardiovascular > Atherosclerosis > Diabetes associated

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism

    Metabolism > Types of disease > Cancer

    Metabolism > Types of disease > Diabetes

    Metabolism > Types of disease > Heart disease

    Signal Transduction > Antibodies > glucose transporter glut1

    Signal Transduction > Metabolism > Plasma Membrane > Channels

    亚细胞定位Cell membrane; Multi-pass membrane protein. Melanosome. Note=Localizes primarily at the cell surface. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    组织特异性Expressed at variable levels in many human tissues.
    翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
    功能Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
    保存条件Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene encodes a major glucose transporter in the mammalian blood-brain barrier. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {Flow-Cyt}{1μg/Test}
    {IF}{1:100-500}

     

    产品细节图片1
    Blank control: HepG2.
    Primary Antibody (green line): Rabbit Anti-GLUT1 antibody (bs-0472R-FITC)
    Dilution: 2μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
    产品细节图片2
    Blank control:U87MG.
    Primary Antibody (green line): Rabbit Anti-GLUT1 antibody (bs-0472R-FITC)
    Dilution: 2μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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