GPER1 | GPR30 Rabbit pAb, FITC conj.(bs-1380R-FITC)-100ul产品图

GPER1 | GPR30 Rabbit pAb, FITC

conj.(bs-1380R-FITC)-100ul
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  • bs-1380R-FITC
  • 2026年10月17日
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    • 规格:

      100ul

    产品编号bs-1380R-FITC
    英文名称GPR30 Rabbit pAb, FITC conjugated
    中文名称FITC标记的G蛋白偶联受体30抗体
    英文别名G Protein Coupled Receptor 30; G-protein coupled receptor 30; G-protein coupled estrogen receptor 1; Membrane estrogen receptor; mER; Chemokine receptor-like 2; IL8-related receptor DRY12; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; Lymphocyte-derived G-protein coupled receptor; LYGPR; GPCR-BR; CEPR; CMKRL2; DRY12; GPER.
    产品应用Flow-Cyt=1µg/Test, IF=1:100-500, ICC/IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human GPR30
    亚型IgG
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Estrogen

    Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism

    Neuroscience > Neurotransmission > Receptors / Channels > GPCR > More GPCR

    Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR

    亚细胞定位Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Golgi apparatus membrane; Multi-pass membrane protein (By similarity). Note=Protein has been detected in the cell membrane, endoplasmic reticulum and Golgi apparatus. It is currently unclear whether this is a cell surface or intracellular receptor.
    组织特异性Ubiquitously expressed, but is most abundant in placenta. In brain regions, expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.
    相似性Belongs to the G-protein coupled receptor 1 family.
    功能Receptor for estrogen.
    保存条件Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {Flow-Cyt}{1µg/Test}
    {IF}{1:100-500}
    {ICC/IF}{1:100-500}

     

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    图标文献和实验
    相关实验
    • The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro

      reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL3331) (for Protocol 3) ESC medium (for Protocols 1, 2) Freezing

    • 抗原抗体反应的应用

      (常为IgG)组分。常用的纯化IgG的方法为饱和硫酸胺盐析和层析法。蛋白质在不同的盐浓度的溶液中,其溶解度不一样,盐离子干扰蛋白质和水分子间氢键形成,因为水—盐结合比水—蛋白质结合更稳定,蛋白质即可从溶液中沉淀出来。蛋白质分子越大,沉淀时所需盐离子浓度越低。免疫球蛋白(Mr1.5×105)比血清中主要蛋白质白蛋白(Mr6.7×104)的分子大得多,抗体在30%—50%饱和度的硫酸盐中析出,而白蛋白需在70%—80%饱和度才析出,因此常用33%饱和度的硫酸胺纯化血清中的IgG.盐析时为了减少抗体

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