Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Human Hela cell lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human Jurkat cell lysates
Lane 7: Human Huvec cell lysates
Primary: Anti-Beta tubulin/HRP (bsm-33034M-HRP) at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
- ￥1200
- Bioss
- bsm-33034M-HRP
- 2025年10月15日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bsm-33034M-HRP |
| 英文名称 | Beta tubulin Mouse mAb, HRP conjugated |
| 中文名称 | HRP标记的微管蛋白β tubulin/Tubulin β（内参）单克隆抗体 |
| 英文别名 | beta 4 tubulin; beta 5 tubulin; beta Ib tubulin; beta1 tubulin; Class I beta tubulin; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SWcl.56; TBB5_HUMAN; TUBB 1; TUBB 2; TUBB 5; TUBB; TUBB1; TUBB2; TUBB5; tubulin beta 1 chain; Tubulin beta 2 chain; tubulin beta 5 chain; Tubulin beta chain; Tubulin beta class I; tubulin beta polypeptide; Tubulin beta-5 chain. |
| 产品应用 | WB=1:5000-50000, IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from human Beta tubulin |
| 亚型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microtubules > Tubulin Tags & Cell Markers > Subcellular Markers > Cytoskeleton > Microtubules |
| 亚基 | Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1. |
| 亚细胞定位 | Cytoplasmic, cytoskeleton. |
| 组织特异性 | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). |
| 相似性 | Belongs to the tubulin family. |
| 功能 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:5000-50000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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Beta tubulin Mouse mAb, HRP conjugated(bsm-33034M-HRP)-100ul
￥1200
