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产品信息以Bioss网站为准
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100ul
| 产品编号 | bs-1571R-FITC |
| 英文名称 | F-Actin Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的纤维状肌动蛋白抗体 |
| 英文别名 | f actin; Filamentous actin; F-actin capping protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1. |
| 产品应用 | Flow-Cyt=3μg /test, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | Synthetic MAP peptide derived from human beta-Actin |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Isotype/Loading Controls > Loading Controls > Beta Actin Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Tags & Cell Markers > Subcellular Markers > Cytoskeleton > Actin |
| 亚基 | Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 组织特异性 | Ubiquitously expressed in all eukaryotic cells. |
| 翻译后修饰 | ISGylated. Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced. |
| 相似性 | Belongs to the actin family. |
| 功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {3μg /test} |
| {IF} | {1:100-500} |
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文献和实验[IF={{ 4.1 }}] {Du et al. Actin filament reorganization is a key step in lung inflammation induced by systemic inflammatory response syndrome. (2012) Am.J.Respir.Cell.Mol.Biol. 47:597-603} {IF} {Rat}
Fluorochrome Absorption and Emission Spectra
conjugates to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting
Fluorochrome Absorption and Emission
to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting light at 488 nm
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL3331) (for Protocol 3) ESC medium (for Protocols 1, 2) Freezing
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