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- 详细信息
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- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
100ul
| 产品编号 | bs-5849R-HRP |
| 英文名称 | ADAM18 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的去整合素样金属蛋白酶18抗体 |
| 英文别名 | A disintegrin and metalloproteinase domain 18; ADAM 18; ADA18_HUMAN; ADAM 27; ADAM metallopeptidase domain 18; ADAM27; MGC41836; MGC88272; TMDC3; tMDCIII; Transmembrane metalloproteinase like, disintegrin like, and cysteine rich protein III. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ADAM18 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Expressed specifically in testis. |
| 翻译后修饰 | The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa. |
| 相似性 | Contains 1 disintegrin domain._x000D_
Contains 1 EGF-like domain._x000D_ Contains 1 peptidase M12B domain. |
| 功能 | Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | ADAM18 was first described as tMDC III (testis-specific Metalloproteinase-like disintergin-like cysteine-rich protein-III). ADAM18 is a member of the ADAMs family (A Disintegrin And Metalloproteinase), but does not contain the canonical HExxHxxxxH zinc-binding metalloproteinase catalytic site. The domain structure of the fill-length ADAM18 includes a signal sequence, propeptide domain, metalloproteinase-like domain, disintegrin-like domain, cys-rich domain, EGF-like domain, a short spacer region, then the transmembrane domain and a cytoplasmic domain. Like many of the reproductive-specific ADAMS, ADAM18 plays a nonenzymatic role, or (as is the case for ADAMs 1 & 2 (fertilin alpha and beta)), the protein acts in concert with a proteolytically active ADAM to process proteins. Little is known about interactions between ADAM18 and other ADAMs. To date, 4 different sequences for human ADAM18 are published; 739, 715, 182 and 153 amino acids in length. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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